

BED File Size Estimator
This tool calculates the base pair coverage for each chromosome from pasted BED file text. It outputs the results in an HTML table that displays, for each chromosome:
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Coverage (bp): Total base pairs covered.
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Coverage (Mb): Base pairs converted to megabases.
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Coverage (%): The percentage of the chromosome's length (based on GRCh38 sizes) that is covered.
Key Points:
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Input:
Paste the text content of a BED file (which lists genomic intervals) into the designated input area. -
Output:
A neatly formatted table with columns for Chromosome, Coverage (bp), Coverage (Mb), and Coverage (%) is displayed. -
Default Chromosome Order:
The tool shows results for the default human chromosomes (chr1–chr22, chrX, chrY, and chrM), listing all even if no intervals are found for some chromosomes. -
Important Limitation:
The calculation does not account for overlapping regions. If two or more intervals overlap, the overlapping region will be counted multiple times—once for each interval that covers it.
This tool is ideal for quickly estimating coverage based on BED file data, but users should be aware of the overlap limitation when interpreting the results.
Output
Reference
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Sims, D., Sudbery, I., Ilott, N. E., Heger, A., & Ponting, C. P. (2014). Sequencing depth and coverage: key considerations in genomic analyses. Nature Reviews Genetics, 15(2), 121-132. https://doi.org/10.1038/nrg3642