CHANDLER HO, MHA
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About me
I am an ASCP board-certified medical laboratory scientist and health care leader passionate about translating and integrating innovations to laboratory medicine with clinical standards. My expertise includes test development, clinical laboratory operations, regulatory compliance, reimbursement, and quality management system within CLIA- and CAP-accredited settings.
Tools
Reverse Complement
A reverse complement tool takes a DNA sequence, replaces each base with its complement, , then reverses the result to give the opposite strand in 5′→3′ orientation.
Illumina Coverage Estimator
This estimator is designed to assist researchers in planning and optimizing their sequencing experiments by providing a quantitative assessment of the data required per sample.
GC Content
GC content is the percentage of nucleotides in a DNA or RNA sequence that are guanine (G) or cytosine (C); it affects melting temperature, stability, and often correlates with genomic features like gene density and PCR/primer design.
BED File Size Estimator
This tool calculates base pair coverage for each chromosome from pasted BED text and outputs an HTML table showing Coverage (bp), Coverage (Mb), and Coverage (%) with the percentage computed relative to GRCh38 chromosome lengths.
Lymphotrack Normalization
This library normalization calculator is designed for a seven-target Lymphotrack assays.
Lymphotrack Diversity
This script calculates Shannon's E for lymphotrack file unique clonotypes.
Rebalance Illumina Libraries
This use low-pass index% data to rebalance libraries for whole-genome sequence.
Publications
Hammond N, et al. Analytical validation of germline small variant detection using long-read HiFi genome sequencing. Genome Res. 2025;35(6):1391–1399. PMID: 40216554.
Pollack AS, et al. Immune repertoire profiling uncovers pervasive T-cell clonal expansions in benign prostatic hyperplasia. J Clin Invest. 2025;135(11):e186939. PMID: 40178915.
Wiredja D, et al. Performance evaluation of a next-generation sequencing–based T-cell receptor gene rearrangement assay. J Mol Diagn. 2025;27(6):465–474. PMID: 40158883.
Hammer PM, et al. POLE-mutated uterine carcinosarcomas: a clinicopathologic and molecular study of 11 cases. Mod Pathol. 2025;38(3):100676. PMID: 39615841.
McHenry A, et al. Molecular classification of metastatic and recurrent endometrial endometrioid carcinoma: prognostic relevance among low- and high-stage tumours. Histopathology. 2024;85(4):614–626. PMID: 38859768.
Hammer PM, et al. Molecular classification outperforms histologic classification in prognostication of high-grade endometrial carcinomas with undifferentiated and sarcomatous components. Am J Surg Pathol. 2024;48(8):953–964. PMID: 38780000.
Fei F, et al. Diagnostic impact of RNA-based next-generation sequencing fusion panel for solid tumors: a single-institution experience. Am J Clin Pathol. 2024;161(4):329–341. PMID: 38001052.
Zhang H, et al. Longitudinal study of 2 patients with cyclic thrombocytopenia, STAT3 and MPL mutations. Blood Adv. 2023;7(1):190–194. PMID: 35381066.
Tung JK, et al. Minimal/measurable residual disease monitoring in patients with lymphoid neoplasms by high-throughput sequencing of the T-cell receptor. J Mol Diagn. 2023;25(6):331–341. PMID: 36870603.
Devereaux KA, et al. A multiplex SNaPshot assay is a rapid and cost-effective method for detecting POLE exonuclease domain mutations in endometrial carcinoma. Int J Gynecol Pathol. 2022;41(6):541–551. PMID: 34907997.
Devereaux KA, et al. Prospective molecular classification of endometrial carcinomas: institutional implementation, practice, and clinical experience. Mod Pathol. 2022;35(5):688–696. PMID: 34743187.
Ho CC, et al. Validation of a next-generation sequencing–based T-cell receptor gamma gene rearrangement diagnostic assay: transitioning from capillary electrophoresis to next-generation sequencing. J Mol Diagn. 2021;23(7):805–815. PMID: 33892183.
Gu W, et al. Detection of cryptogenic malignancies from metagenomic whole genome sequencing of body fluids. Genome Med. 2021;13(1):98. PMID: 34074327.
Scott GD, et al. Interfacing complex laboratory instruments during a change to Epic Beaker. J Pathol Inform. 2018;9:24. PMID: 30034922.
Book Chapter
Wadsworth, P. A., Ho, C. C., & Zhang, B. M. (2024). Immunoglobulin and T‐Cell Receptor Gene Assessment. Manual of Molecular and Clinical Laboratory Immunology (9th edition), 1, 23-38.